Fig. 8.

Effect of peritoneal dialysis fluid (PDF) on C3 secretion. Quiescent HPMCs were incubated with serum-free M199 medium, or glucose-free PDF, or PDF of various glucose strengths, or equivalent solutions supplemented with 10% FBS for 4 h or 24 h. Culture supernatants were harvested for C3 ELISA. The glucose concentrations of 1·5%, 2·5%, and 4% solutions are 83 mm, 138 mm, and 220 mm, respectively. Results were obtained from triplicate experiments. *P = 0·018, #P = 0·002, †P = 0·000, ‡P = 0·011, §P = 0·001 compared to medium control.