Fig. 12.

Effect of peritoneal dialysis fluid on LDH release. Quiescent HPMCs were incubated with serum-free M199 medium, or glucose-free PDF, or PDF of various glucose strengths (a), or equivalent solutions supplemented with 10% FBS (b) for 1 h (⋄), 4 h (○), or 24 h (•). Culture supernatants were harvested for assay of LDH release. The glucose concentrations of 1·5%, 2·5%, and 4% solutions are 83 mm, 138 mm, and 220 mm, respectively. Results were obtained from triplicate experiments. *P = 0·001, #P = 0·005, †P = 0·000 compared to medium control.