Table 3.

Inhibition of monoclonal antibody binding to TPO by rabbit antipeptide antibodies and pooled Graves’ patients’ serum positive for anti-TPO antibodies

	IDR-B region specific mabs (% inhibition *)			IDR-A region specific mabs (% inhibition *)
Antibodies	mab 15	mab 18	mab 64	mab 2	mab 47
Pre-immune	4	5	5	6	4
P12	5	6	11	20	0
P14	97	93	86	3	1
P18	6	7	8	17	2
P43	4	10	10	27	99
P12 + P14	100	100	87	48	5
P18 + P14	100	100	93	22	8
P14 + P43	100	100	95	40	92
P12 + P18	10	13	11	45	12
P12 + P43	12	24	18	70	95
P18 + P43	10	20	15	46	96
P12 + P14 + P18	100	100	92	75	17
P12 + P14 + P43	100	100	93	78	99
P18 + P14 + P43	100	100	98	50	98
P12 + P14 + P18 + P43	100	100	98	72	99
P4 + P30 + P32	10	14	13	11	7
Blood donor serum pool	0	0	1	2	0
Graves’ patients’ serum pool (n = 20)	97	96	77	82	14

^*standard deviation for all results in Table 1 were between 4 and 8%.