Table 2.
Pulmonary inflammatory and IgE responses from HDE with equivalent cockroach allergen levels. Mice were immunized and challenged with HDE diluted 1 : 10, Imm (1 : 10), or containing equivalent Bla g levels, Imm (equiv.); 48 h after the last pulmonary challenge mice were sacrificed and BAL fluid was collected. Differential counts were performed by counting 300 cells. Total plasma IgE was measured by ELISA as before
| Total cells/mouse × 104 | Eos./mouse × 104 | Lymphs./mouse × 104 | Macs./mouse × 104 | PMNs/mouse × 104 | IgE (ng/ml) | |||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Imm (1 : 10) | Imm (equiv.) | Imm (1 : 10) | Imm (equiv.) | Imm (1 : 10) | Imm (equiv.) | Imm (1 : 10) | Imm (equiv.) | Imm (1 : 10) | Imm (equiv.) | Imm (1 : 10) | Imm (equiv.) | |
| 1 | 53·9 | 12·2* | 5·3 | 0·1 | 8·0 | 0·4* | 16·5 | 10·4 | 24·1 | 1·3* | 203 | 90 |
| 2 | 21·0 | 6·8 | 1·8 | 0·0 | 5·4 | 0·1 | 10·0 | 6·4 | 5·0 | 0·3 | 94 | 85 |
| 3 | 18·7 | 6·7* | 1·1 | 0·3 | 2·8 | 0·4* | 8·7 | 5·3 | 6·0 | 0·7* | 56 | 46 |
| 4 | 77·0 | 15·2* | 6·3 | 0·1 | 14·5 | 1·4* | 22·5 | 10·7 | 32·0 | 2·9* | 175 | 66 |
| 5 | 77·6 | 18·1* | 6·1 | 0·2 | 15·7 | 0·4* | 33·6 | 13·6* | 22·2 | 3·8* | 389 | 73* |
*
P < 0·05 compared to mice immunized and challenged with the same HDE diluted 1 : 10. Each value is the mean for n = 4, equivalent bla g groups, n = 7 HDE (1 : 10), except HDE 4 (1 : 10), n = 4 due to limited supply. PMN: polymorphonuclear cell.