Fig. 3.

(a) Expression of Iα germline transcripts in unstimulated PBMCs. Semiquantitative determination using RT-PCR analysis. In each case, 28, 32, 36 and 40 cycles were run. β-actin was used as a control with a run of 28, 32 and 36 cycles. The position of target cDNA is indicated by an arrow. C1 and C2, normal controls; P1–P6, patients 1–6. (b) Competitive PCR of the expression of Iα germline transcripts. Each template contained the same amount of cDNA synthesized from RNA extracted from PBMCs after stimulation with PMA and TGF-β1 and one of fivefold dilutions of Iα germline transcript competitor (lanes 1–5). Each equivalent point is indicated by an arrow. C1 and C2, normal controls; P1–P6, patients 1–6.