Fig. 5.
Expression of Cα mature transcripts in PBMCs cultured without (a) or with (b) PMA and TGF-β1. Semiquantitative determination using RT-PCR analysis. In each case, 32, 34, 36 and 38 cycles were run. β-actin was used as a control with a run of 28, 32 and 36 cycles. The positions of target cDNA are indicated by arrows. C1 and C2, normal controls; P1–P6, patients 1–6. (c) IgA secretion was induced by activation of PBMCs. PBMCs (106/mL) were cultured in the presence or absence of anti-CD40, IL-4, and IL-10 for seven days. Concentration of IgA in the supernatant of PBMCs was measured by an enzyme-linked immunosorbent assay. P1–P3, patients 1–3.