Fig. 2.

Immunoblot analysis of cell fractions for cytochrome c and COX 4. Data are presented from duplicate experiments (experiment 1 lanes 2, 4, 6, 8; experiment 2 lanes 3, 5, 7, 9). (a) Mitochondrial (lanes 2–5) and cytoplasmic (lanes 6–9) fractions from non-apoptotic control cells (C; lanes 2–3 and 6–7) and apoptotic (A; lanes 4–5 and 8–9) probed with anticytochrome c antibody. The positive control lane 1 contained 1 µg cytochrome c. The membrane was subsequently stripped and (b) reprobed with anti-COX 4 antibody.