FIG. 1.
Mash-2 protein levels, which decline in human trophoblast cells upon differentiation in a 20% O2 environment and are maintained in trophoblast cells cultured in a hypoxic environment, are primarily localized to the cytoplasm. (A) Immunoblot analysis of hMash-2 protein in nuclear and cytosolic fractions (20 μg/lane) from human cytotrophoblasts before culture (Cyto) and after 3 days of culture in a 20% O2 environment (Syn) (left panel) or in human trophoblasts cultured in either 2 or 20% O2 for 3 days (right panel). C, cytoplasmic; N, nuclear. (B) Immunofluorescence staining of hMash-2 protein in freshly isolated cytotrophoblasts (Cyto) or in trophoblast cells cultured for 3 days in either a 2 or a 20% O2 environment.