FIG. 5.

Mash-2 overexpression increases binding activities of USF1 and USF2 proteins for the E2 and E3 boxes upstream and within hCYP19 exon I.1. Freshly isolated cytotrophoblasts were infected with recombinant adenoviruses expressing either Mash-2 (CMV-Mash-2; MOI = 5) or β-Gal (CMV-β-Gal; MOI = 5) and cultured for 3 days in a 20% O₂ environment. Nuclear proteins isolated from the cultured cells were incubated with ³²P-labeled oligonucleotides containing the E1, E2, and E3 boxes in the absence (−) or presence (+) of antibodies specific for USF1 and USF2 and analyzed by EMSA.