Fig. 6.

Recognition of alkylamine and alkylphosphate antigens by Vγ9Vδ2 T cells requires contact with cells of human origin. Mean number of spots in IFNγ ELISpot assay with and without antigen presenting cells. 1000 γδ T cells/well were plated with 25 000 EBV-transformed human B cells, Spinner Hela cells, rat myeloma Y3.AG.1.2.3 or mouse myeloma P3/NS1/1-Ag4·1. Only human cells were able to act as antigen presenting cells in this assay. The use of 25 000 γδ cells allowed cell-cell contact and confirms that these cells have some ability to present alkylphosphate and alkylamine antigens to each other. Secbutylamine and isobutylamine were added to assays at 10 mm; IPP was added at 100 µm. Assays were performed over 12 h as described in the Materials and Methods. Standard deviation from the mean of four replicates is shown.