Table 2.
Percentages and absolute numbers of NK1·1+/αβ-TCR cell subsets in liver from mock-infected, L2-MHV3- and YAC-MHV3-infected C57BL/6 mice
| % NK1·1+ cell subsets (absolute number ×105)2 | |||||
|---|---|---|---|---|---|
| Viral infection | Hepatitis1 | NK1·1+TCR- | NK1·1+TCRinter | NK1·1−TCRinte | NK1·1−TCRhigh |
| Mock-infected | – | 5·3 ± 1·4 | 17·0 ± 1·5 | 9·4 ± 1·2 | 8·1 ± 0·3 |
| (1·00 ± 0·27) | (3·21 ± 0·28) | (1·78 ± 0·23) | (1·53 ± 0·06) | ||
| L2-MHV3 | + + + | 6·5 ± 2·8 | 8·4 ± 0·9** | 20·1 ± 0·4** | 7·0 ± 1·4 |
| (5·43 ± 2·34)** | (7·01 ± 0·75)** | (16· ± 0·33)** | (5·8 ± 1·17)** | ||
| YAC-MHV3 | + | 5·1 ± 1·9 | 9·3 ± 0·5*** | 12·1 ± 1·4 | 5·7 ± 2·3 |
| (1·49 ± 0·56) | (2·72 ± 0·15) | (3·54 ± 0·41)* | (1·67 ± 0·67) | ||
Hepatitis was determined by a macroscopic examination of liver: + no discoloration, small necrotic foci; + ++ discolouration, extensive necrotic foci; and high friability.
Intrahepatic lymphocytes were purified by Percoll gradient, double-labelled with anti-NK1·1−PE and anti-TCR-FITC MoAbs and analysed using a FACScan flow cytometer. Lymphoid cells were gated according to FSC/SSC parameters and cell numbers were evaluated based on a total of 10 000 recorded events. Absolute numbers were calculated by comparing the percentage of cells in each region with the total number of cells obtained from liver.
P < 0·05;
P < 0·01;
P < 0·001.