Table 3. Comparison of mRNA cytokine levels in unstimulated single-cell suspensions taken at 5 or 14 days postsqualene injection. Lymph node cells from 18 rats were pooled at each time-point, respectively.
| Pre-conA cytokine levels | ||
|---|---|---|
| 5 | 14 | |
| IL-1β/GAPDH | ||
| Axillary/brachial | 0·001 | 0·089 |
| Inguinal | 0·185 | 1·140 |
| IFN-γ/GAPDH | ||
| Axillary/brachial | 0·002 | 0·021 |
| Inguinal | 0·028 | 0·184 |
| IL-4/GAPDH | ||
| Axillary/brachial | 0·867 | 1·891 |
| Inguinal | 1·530 | 0·310 |
| IL-13/GAPDH | ||
| Axillary/brachial | U.V. | 0·194 |
| Inguinal | U.V. | 0·098 |
| IFN-γ/IL-4 | ||
| Axillary/brachial | 0·002 | 0·011 |
| Inguinal | 0·019 | 0·594 |
| IFN-γ/IL-13 | ||
| Axillary/brachial | N.D. | 0·106 |
| Inguinal | N.D. | 1·878 |
N.D: not determined; UV: undetectable values