Preferential expression of CD9 on CD4+CD45RA+ human naive T cells. CD9 defined by anti5H9 is present on a subpopulation of human peripheral blood T cells. Four-colour staining analysis of freshly isolated human PBMC was performed using FITC-conjugated anti-CD45RO, PE-conjugated anti-CD45RA, PerCP-conjugated anti-CD4, biotinylated anti5H9 and APC-conjugated streptavidin (a) and FITC-conjugated anti-CD45RA, PerCP-conjugated anti-CD4, PE-conjugated anti-CD62L, biotinylated anti5H9 and APC-conjugated streptavidin (b). Numbers indicate the relative percentages of positive cells within a quadrant. The result is representative of five separate experiments.