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. 2004 Jul;137(1):109–116. doi: 10.1111/j.1365-2249.2004.02515.x

Fig. 3.

Fig. 3

Iron protects tumour cells by down-regulating macrophage and tumour cell NO release. (a,b) Murine macrophages were incubated with C6 (a) or L929 (b) cells and stimulated with IFN-γ + LPS in the presence or absence of aminoguanidine (AG; 2 mm) and FeSO4 (0·2 mm ive (b)). (c,d) Confluent L929 (c) or C6 (d) cells were stimulated with IFN-γ + LPS, in the presence or absence of various concentrations of FeSO4. (a–d) Nitrite concentration and cell viability were determined after 48 h. Control value (100%) for NO production represents macrophage or tumour cell NO release in the absence of AG or FeSO4. Control value for the cell viability (100 ± 5·2%) refers to viability of tumour cells incubated in the absence of macrophages or IFN-γ + LPS (c,d); *P < 0·05 refers to cultures without FeSO4.