Fig. 7.

Phosphorylation of IKK and IL-8 reporter gene activation in E. coli-infected cultured cells derived from CD34⁺. (a) The cultured cells (day 14) were incubated with E. coli for the indicated times. The ratio of E. coli to the cells was adjusted to 10 : 1. Phosphorylation and protein expression of IKKα and actin were assessed by immunoblot. (b) Cultured cells (day 14) were transfected with pIL-8-luciferase transcriptional reporters; 48 h later the transfected cells were incubated with E. coli (black bar) or TNF-α (20 ng/ml, open bar) for 6 h. Data are expressed as the mean fold induction ± s.e.m. in luciferase activity relative to non-stimulated controls (n = 5).