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Representative histograms showing frequency of (2) CD4+ T cells producing IFN-γ cytokine and (3) CD8+ T-cells producing IFN-γ, measured by dual staining in flow cytometry in C. trachomatis positive infertile women in comparison to negative/unstained controls (1). FL-1 height was assigned to surface marker (CD4 & CD8) staining using antibodies conjugated with FITC (X-axis) and FL-2 height was assigned to cytokine (IFN-γ) staining using antibodies conjugated with PE (x-axis).
