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. 2004 Sep;137(3):496–502. doi: 10.1111/j.1365-2249.2004.02567.x

Fig. 5.

Fig. 5

Long-term reduction of primed antibody responses in Fabry mice to r-hαGAL induced by acute methotrexate treatment. (a) Groups of five Fabry mice received two weekly injections of r-hαGAL (1 mg/kg) to induce αGAL specific IgG production. These primed mice were given four additional weekly r-hαGAL injections (1 mg/kg) with or without i.v. MTX (0, 10 or 50 mg/kg) 48 h following each r-hαGAL treatment. Serum was collected at the time-points indicated and analysed for αGAL-specific IgG by ELISA. After seven treatments, the mean of the 50 mg/kg MTX group was significantly different (P < 0·05 by Student's t-test) from the 0 mg/kg group. (b) At week 15, weekly r-hαGAL (1 mg/kg) injections were resumed without MTX treatment. At 21 weeks, serum was collected and analysed for αGAL-specific IgG by ELISA. After 21 treatments, the mean of the 50 mg/kg MTX group was significantly different (P < 0·05 by Student's t-test) from the 0 mg/kg group.