FIG. 6.

MafA binds within the enhancer region of the endogenous insulin gene. The cross-linked DNA immunoprecipitated from βTC-3 cells with αMafA antibody was analyzed by PCR with insulin (−378/−46) and PEPCK control region-specific primers (lane 3). As controls, PCRs were run with no DNA (lane 1), on input chromatin (1:100 dilution, lane 2), or with DNA obtained after precipitation with rabbit IgG (lane 4) or no antibody (lane 5). The same pattern was obtained with the Maf#153 antisera (data not shown).