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. 2004 Oct;138(1):14–20. doi: 10.1111/j.1365-2249.2004.02591.x

Fig. 2.

Fig. 2

Macrophage lysate ADA activity: Peritoneal macrophages were lysed after 24 h incubation in vitro. ADA activity was measured and reported as nmoles NH3/ h·104cells. (a) Both ADA1 (Inline graphic) and ADA2 (Inline graphic) were measurable in macrophage lysates of rats, with ADA1 representing the predominant proportion of total ADA activity. Activity of both isozymes were significantly higher in macrophages from rats with peritonitis. (b) Activity attributable to ADA2 represented < 50% of the total activity, regardless of rat condition. *Significantly different relative to nonseptic naive values. P < 0·05. Samples sizes (n) were 4 for nonseptic (each sample representing the pooled cells from 2 to 3 rats) and 6 for septic groups, each sample representing macrophages from a single septic rat.