Fig. 4.

Peripheral blood monocyte lysate ADA activity: Peripheral blood monocytes were lysed after 24 h incubation in vitro. ADA activity was measured and reported as nmoles NH₃/ h·10⁴cells. (a) Both ADA1 () and ADA2 () were measurable in monocyte lysates of rats, with ADA1 representing the predominant proportion of total ADA activity. However, cell concentrations were 50–200-fold lower than that of macrophages (note axis scale difference compared to Fig. 2). Activity of only ADA2 was significantly higher in monocytes from rats with peritonitis. (b) Activity attributable to ADA2 was significantly higher in monocytes collected from rats with peritonitis. *Significantly different relative to nonseptic naive values. P < 0·05. Samples sizes (n) were 4 for nonseptic (each sample representing the pooled cells from 2 to 3 rats) and 6 for septic groups, each sample representing macrophages from a single septic rat.