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. 2004 Oct;138(1):14–20. doi: 10.1111/j.1365-2249.2004.02591.x

Fig. 5.

Fig. 5

Peripheral blood monocyte media ADA activity: Peripheral blood monocytes were incubated with RPMI media supplemented with 10% HS and 5% PSN for 24 h. Media was collected and subsequently assayed for ADA activity. (a) Both ADA1 (Inline graphic) and ADA2 (Inline graphic) were measurable in monocyte media. In nonseptic rats, ADA1 represented the predominant proportion of total ADA activity. Only ADA2 activity was higher in the media collected from septic monocytes. (b) Activity attributable to ADA2 was significantly higher in media of monocytes collected from rats with peritonitis. *Significantly different relative to nonseptic naive values. P < 0·05. Samples sizes (n) were 4 for nonseptic (each sample representing the pooled cells from 2 to 3 rats) and 6 for septic groups, each sample representing macrophages from a single septic rat.