FIG. 10.

Nonacetylatable histone H4 mutants are hypersensitive to benomyl. Wild-type and mutant cells at the same cell density in YPD were spotted in 10-fold serial dilutions onto YPD plates without benomyl (YPD) or with 15 μg of benomyl/ml (Ben), followed by incubation at 28°C for 3 days. For each mutant allele, the amino acid substitutions at positions 5, 8, 12, and 16 or the N-terminal tail are indicated as K (lysine), Q (glutamine), or R (arginine). Allele hhf1-25 contains an insertion of G-K-G at position 3 of the hhf1-10 tail (designated <K>QQQQ), whereas allele hhf1-35 contains an insertion of G-K-G at position 12 of the hhf1-10 tail (designated QQQ<K>Q). Both hhf1-10 and hhf1-34 are roughly 5 orders of magnitude hypersensitive to benomyl and are the only mutants that lack any acetylatable lysine in their histone H4 tail.