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. 2003 Sep;23(17):6086–6102. doi: 10.1128/MCB.23.17.6086-6102.2003

FIG. 3.

FIG. 3.

yaf9Δ is synthetically lethal with SPB and kinetochore mutants and it is hyper-sensitive to benomyl. (A) yaf9Δ is synthetically lethal with the bbp1-1 mutant. yaf9Δ (ILM162), bbp1-1 (YCS64), and yaf9Δ bbp1-1 (ILM113) cells containing the BBP1 URA3 plasmid (pli36) were streaked at 24°C, the permissive temperature for the bbp1-1 mutant, onto plates without uracil (−URA) or onto plates containing 5-FOA to counterselect the URA3 plasmid. The absence of growth on the 5-FOA plate of the yaf9Δ bbp1-1 (ILM113) cells (double in the figure) shows that the yaf9Δ bbp1-1 double mutant cannot grow in the absence of the BBP1 URA3 plasmid. (B) yaf9Δ is synthetically lethal with the spc24-11 mutant. yaf9Δ (ILM162), spc24-11 (ILM128), and yaf9Δ spc24-11 (ILM140) cells containing the SPC24 URA3 plasmid (pli39) were streaked at 24°C, the permissive temperature for the spc24-11 mutant, onto plates without uracil (−URA) or onto plates containing 5-FOA to counterselect the URA3 plasmid. The absence of growth on the 5-FOA plate of the yaf9Δ spc24-11 (ILM140) cells (double in the figure) shows that the yaf9Δ spc24-11 double mutant cannot grow in the absence of the SPC24 URA3 plasmid. (C) The yaf9Δ mutant is hypersensitive to benomyl. Wild-type (YPH499) and yaf9Δ (ILM162) cells at the same cell density in YPD were spotted in 10-fold serial dilutions onto YPD plates containing the indicated concentrations of benomyl, and the plates were subsequently incubated at 24°C for 3 days. WT, wild type.