FIG. 5.

MTs depolymerize more readily and recover more slowly from limiting nocodazole in the yaf9Δ mutant compared to the wild type. Wild-type (CMY1228) and yaf9Δ cells (CMY1229) expressing GFP-Tub1 were grown in YPD at 30°C and treated with nocodazole at the concentrations and for the time periods indicated in the figure. (A) The percentage of wild-type and yaf9Δ cells with no detectable GFP-Tub1 foci after treatment with the indicated concentrations of nocodazole was quantified. A fluorescent GFP-Tub1 focus is found at the SPB of cells containing polymerized tubulin. The absence of such foci is thus an indication of an essentially complete depolymerization of MTs. (B) Images illustrating the different responses of wild-type and yaf9Δ cells to incubation with 3 μg of nocodazole/ml for 3 h. WT, wild type.