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. 2003 Sep;23(17):6086–6102. doi: 10.1128/MCB.23.17.6086-6102.2003

FIG. 9.

FIG. 9.

The esa1-1851 and yng2Δ mutants are hypersensitive to benomyl, and the HDAC inhibitor TSA differentially affects the growth and benomyl resistance of wild type and NuA4 HAT mutants. (A and B) Wild type (W303-1a) and esa1-1851 (MSY2431) and esa1-L357H (MSY2432) mutants were spotted onto either YPD alone or YPD plus 15 μg of benomyl/ml at 30°C for 3 days. (C to F) Tenfold serial dilutions of exponentially growing yng2Δ (CMY1237), wild-type (W303-1a), and yaf9Δ (ILM63) cells in YPD at the same cell density were spotted onto plates containing the indicated media and incubatedat 24°C for 1 day (A and B) or 4 days (C and D). YPD+TSA is YPD containing TSA at 30 μg/ml, YPD+Ben is YPD containing benomyl at 10 μg/ml, YPD+Ben+TSA is YPD containing benomyl at 10 μg/ml and TSA at 30 μg/ml. (G) Wild-type (W303-1a) cells were spotted on the indicated media and incubated at 24°C for 2 days. WT, wild type.