Fig. 1.
IL-8 production by infected human alveolar lung epithelial cell line (A549). The cells (5 × 105 cells/well) were infected for 2 h with either B. pseudomallei or S. typhi at MOI of 0·1 : 1, 1 : 1, 10 : 1 and 100 : 1. The infected cells were washed with PBS three times before fresh culture medium containing 250 µg/ml of kanamycin was added and cultured for 8 h. The supernatants were collected and used for IL-8 analysis by ELISA. Data represent mean and standard errors of 3 separate experiments, each carried out in duplicate. *P ≤ 0·01 by Student's t-test.