Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.
Effects of cyclohexamide on the expression of IL-8 mRNA in B. pseudomallei-infected cells. The cells were pretreated with cyclohexamide (5 µg/ml) for 2 h before being infected with the bacteria at MOI of 10 : 1. After 2 h of incubation, the infected cells were washed with PBS and then lysed. The expression of IL-8 mRNA was determined by RT-PCR as described in Materials and methods.
