Table 1.
Primary monoclonal antibodies used for immunohistochemistry
| MoAb and specificity | Blocking serum | Dilution | Incubation time | Incubation temperature | Source | Clone |
|---|---|---|---|---|---|---|
| IL-8 | Horse | 1/100 | overnight | 4°C | Biosource1 | 893C4G2 |
| IL-1β | Horse | 1/20 | overnight | 4°C | R&D2 | 8516·331 |
| IL-8RA (CXCR-1) | Horse | 1/40 | overnight | 4°C | R&D | 42705·111 |
| IL-8RB (CXCR-2) | Human | 1/40 | overnight | 4°C | R&D | 48311·211 |
| CD14; macrophage | Horse | 1/10 | 60 min | Room temperature | Novocastra3 | M-M-42 |
| CD15; granulocyte | Horse | 1/10 | 60 min | Room temperature | Novacastra | BY87 |
| CD3; T lymphocyte | Horse | 1/200 | 60 min | Room temperature | Novocastra | UCHT1 |
1
Biosource International, Camarillo California, USA.
2
R&D Systems, Minneapolis, USA.
3
Novocastra Laboratories Ltd, Newcastle upon Tyne, UK.