Fig. 1.

Hemin-induced HO-1 expression in the spleen and kidney. HO-1 expression is determined by immunoblotting technique. Splenocytes from BALB/c (a) and MRL/lpr (b) were treated in vitro with or without 100 µm hemin for 24 h. The spleens (c) and kidneys (d) were recovered from MRL/lpr mice at 24 h after receiving a single intraperitoneal injection with hemin (100 µmol/kg) or PBS. All samples were obtained from mice at 16 weeks of age. Representative results of more than three individual experiments are shown. Both in vitro and in vivo treatment with hemin led to remarkable enhancement of HO-1 expression in the spleen and kidney.