Table 1.
The effect of stimulator : responder ratio in primary xenogeneic MLR on the efficacy of BTI-322 in inducing hyporesponsiveness during a secondary MLR to cells of the orginal xenogeneic SLA haplotype, to third-party xenogeneic stimulator cells or to allogeneic stimulator cellsa
| Primary xenogeneic MLR (SLAcc stimulation) | ||||
|---|---|---|---|---|
| Proliferation (cpm) | Secondary MLR (SLAcc, SLAdd, or allogeneic stimulation) | |||
| Responder : stimulator | Control Ig | BTI-322 | Stimulator | BTI-322 in primary culture (% of cpm of control Ig) |
| 1 : 1 | 101 500 | 1400 | SLAcc | 3 |
| SLAdd | 2 | |||
| Allogeneic | 5 | |||
| 1 : 0·5 | 56 600 | 1000 | SLAcc | 3 |
| SLAdd | 470 | |||
| Allogeneic | 490 | |||
| 1 : 0·25 | 22 000 | 700 | SLAcc | 3 |
| SLAdd | 40 | |||
| Allogeneic | 180 | |||
| 1 : 0·125 | 4 800 | 700 | SLAcc | 4 |
| SLAdd | 410 | |||
| Allogeneic | 370 | |||
| 1:0 | 400 | 500 | SLAcc or SLAdd | NA |
| Allogeneic | NA | |||
The concentration of responder cells in the primary MLR was 1 × 106/ml, and xenogeneic SLAcc stimulator cells were used at varying responderstimulator ratios. Proliferation data are [3H]TdR incorporation (cpm) at day 5 of the primary MLR in the presence of BTI-322 or control Ig. After harvest and a 3-day culture without stimulation, a secondary MLR was performed with xenogeneic stimulator cells (original SLAcc haplotype or third-party SLAdd), or with allogeneic cells at a 1 : 1 responder : stimulator ratio. The response of cells from primary culture in the presence of BTI-322 is presented as a percentage of that from primary culture in the presence of control Ig: due to the variability in primary and secondary responses this percentage shows variability for the various responder : stimulator ratios.