Table 2.
Effect of F(antibody′)2 fragments of BTI-322 in primary stimulation on unresponsiveness in secondary anti-TCR Vβ stimulationa
| Secondary culture cpm ± s.e.m. (within brackets percentage of control antibody in primary culture) | ||
|---|---|---|
| Antibody supplementation in primary culture with anti-TCR Vβ8 antibody | Anti-Vβ8 | Anti-Vβ13 |
| Control antibody | 20 900 ± 3000 (100) | 20 600 ± 500 (100) |
| BTI-322 | 2700 ± 300 (13) | 17 700 ± 200 (86) |
| BTI-322 F(ab′)2 | 39 000 ± 600 (190) | 61 400 ± 2500 (300) |
a
The primary culture was performed for PBMCs (1 × 106/ml) with anti-Vβ8 antibody and BTI-322 or control Ig, all at 100 ng/ml, for 7 days. After a subsequent 3-day culture without stimulant, a secondary culture was performed with anti-Vβ8 or Vβ13 antibody: [3H]-TdR incorporation was measured after 3 days.