Fig. 4.

Cl^– channel blockers inhibit the chemotactic migration of monocytes. Freshly isolated human monocytes were used for chemotactic migration in 48-well Boyden microchambers. Twenty-six µl of MCP-1 solution (50 ng/ml) was added to the bottom wells of a chemotaxis chamber and then 50 µl of the cell suspension (1–1·5 × 10⁶ cells/ml) was loaded into the top chamber. NPPB (a) or IAA94 (b) in several doses were applied to both bottom and top wells. Experiments were performed in triplicate and the data are shown as mean ± s.e.m. (n = 4). *P < 0·05.