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. 2005 Jan;139(1):65–73. doi: 10.1111/j.1365-2249.2005.02659.x

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© 2005 British Society for Immunology

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Fig. 4 — Chemotactic migration of neutrophils against FMLP. Neutrophils (1 × 10⁵) were allowed to migrate through polycarbonate filters (3 µm) for 90 min at 37°C with CO₂ in response to MEDIUM (control) (▪) or FMLP (10⁻⁸m) (). Then, the filter was fixed and stained and the number of chemotactic cells was counted in five random high-power fields (HPF) × 400 for each of triplicate filters. The data are shown as the Number of PMN/HPF × 400. (a) 24 h (b) 72 h post treatments. *P < 0·001 compared with all groups with MEDIUM, †P < 0·001 compared with all groups; ‡P < 0·001 compared with all groups.

Inline graphic — Chemotactic migration of neutrophils against FMLP. Neutrophils (1 × 10⁵) were allowed to migrate through polycarbonate filters (3 µm) for 90 min at 37°C with CO₂ in response to MEDIUM (control) (▪) or FMLP (10⁻⁸m) (). Then, the filter was fixed and stained and the number of chemotactic cells was counted in five random high-power fields (HPF) × 400 for each of triplicate filters. The data are shown as the Number of PMN/HPF × 400. (a) 24 h (b) 72 h post treatments. *P < 0·001 compared with all groups with MEDIUM, †P < 0·001 compared with all groups; ‡P < 0·001 compared with all groups.