Fig. 2.

mRNA expression of HSP60 and Th1-type cytokines, chemokines and chemokine receptors in the affected sites of intestinal BD. (a) PBL from four BD patients and two HC were analysed for mRNA expression of HSP60 and chemokine receptors. All the BD patients expressed IFN-γ mRNA but did not express IL-4 mRNA. HSP60 was detected in BD PBL but not in HC PBL. The Th1-related chemokine receptor, CCR5, was expressed in all the BD PBL. Another Th1-related chemokine receptor, CXCR3, was detected only in PBL from the patients in the active phase (BD1, BD2). These two patients lacked expression of CCR3, a Th2-related chemokine receptor, whereas it was expressed in BD3 and BD4 in the inactive BD. Another Th2-related chemokine receptor, CCR4, was not expressed in patients BD1 and BD4, whereas it was expressed in BD2 and BD3. (b) Intestinal lesions of four BD patients, four CD patients and one UC patient were analysed. BD patients and CD patients expressed all the Th1-associated mRNAs tested here. In contrast, the UC patient showed CCR4 expression and did not express Th1-associated mRNAs. NC, negative control studies using H2O as templates. Patient BD5 lacked IL-12 mRNA expression probably because she had received high-dose corticosteroid administration at the time of resection.