FIG. 2.

BAF60a links steroid hormone receptors and the BRG1 complex. (A) GST and BAF60a fusion proteins were used to pull down BRG1, BAF170, BAF155, BAF60a, and GR1-556, as described in the legend for Fig. 1C. The relative strengths of the interactions are indicated below the autoradiogram. (B) In vitro pull-down assays de-scribed in the legend for panel A were performed with GR, mutant GR(R488Q), PRb, ERα, FXR, PPARγ1, VDR, and RXRα. Hormones were applied at 1 μM dexamethasone for GR and 10 nM R5020 (PRb), 0.1 μM 17β-estradiol (ERα), 10 μM chenodeoxycholic acid (FXR), 10 μM 15d-prostaglandin J2 (PPARγ1), 10 nM EB1089 (VDR), or 10 μM 9-cis retinoic acid (RXRα) and compared to results for the vehicle without hormone (− Hormone). (C) Sequence alignment of the nuclear receptors used in the pull-down assays to illustrate the RRK motif in the DBD.