Fig. 3.

Expression of calpain II and calpastatin mRNA in skin lesions of psoriasis. Relative mRNA amount of calpain II and calpastatin was assessed by real-time PCR using the ΔΔCt method in lesion skin of psoriasis and normal skin (CTL). Total RNA was isolated from frozen skin tissues, reverse transcribed into cDNA, and then amplified using primers specific for calpain II and calpastatin. One of the control samples was chosen as a calibrator sample. Each histogram shows the mean (± s.d.) results obtained for five patients in each group.