Fig. 4.

Estren inhibits the frequency of CD4⁺ T cells through ERs. Flow cytometry analysis of CD4⁺ cells in spleen of 11-month-old OVX WT and DERKO mice are presented. The mice were given daily s.c. injections of 0·7 µg E2/mouse or 75 µg estren/mouse during 4 weeks. Control mice received vehicle olive oil. Treatment with E2 or estren resulted in lower frequency of CD4⁺ cells in the spleen compared to control mice, while this could not be seen in DERKO mice. One-way anova followed by Fisher's exact test was used to compare data from control mice with E2- or estren-treated mice. **P < 0·01, ***P < 0·001. Results are presented as mean ± standard deviation. n = 5–8 mice in each group.