Table 1.
Typical NCRs-specific fluorescence intensity values obtained with control peripheral blood purified NK cells from healthy donors and used for calculation of MFI values.
Fluorescence intensity | ||||
---|---|---|---|---|
Untreated controls purified NK cells | ||||
Cell type (dot plot quadrant)* | NKp30 | NKp44 | NKp46 | |
Double-stained cells | 115·57 | 137·40 | 141·70 | |
Purified NK cells which do not express NCR | 55·33 | 91·74 | 95·37 | |
Mean** | 60·24 | 45·66 | 56·33 |
Cells were obtained from the upper and lower right quadrants of a dot plot obtained from flow cytometric determinations.
Mean was defined as the difference between the geometric mean of the fluorescence emitted by the purified NK cells that express the specific NCRs analysed (NKp46, NKp44 or NKp30) and the geometric mean of the fluorescence emitted by the purified NK cells that do not express the NCRs (background).