Table 2.
FACS analysis: influence of uraemia on CD4+ T lymphocytes.
| HD patients | PD patients | Predialysis patients | Healthy controls | P-value | |
|---|---|---|---|---|---|
| CD4+ | 37 ± 10 | 50 ± 14 | 58 ± 16 | 42 ± 18 | |
| CD45RA+ | 27 ± 17 | 25 ± 11 | 30 ± 24 | 41 ± 15 | |
| CD45RA+CD25+ | 67 ± 23 | 60 ± 22 | 64 ± 16 | 23 ± 18 | P < 0·001a |
| CD45RA+CD28+ | 92 ± 10 | 96 ± 8 | 94 ± 5 | 97 ± 3 | |
| CD45RA+CCR7+ | 93 ± 9 | 94 ± 5 | 92 ± 6 | 94 ± 5 | |
| CD45RA+CD46+ | 100 ± 1 | 99 ± 2 | 99 ± 1 | 99 ± 1 | |
| CD45RO+ | 73 ± 17 | 75 ± 11 | 71 ± 24 | 59 ± 15 | |
| CD45RO+CD25+ | 74 ± 16 | 76 ± 13 | 76 ± 14 | 54 ± 28 | |
| CD45RO+CD28+ | 91 ± 9 | 94 ± 7 | 93 ± 6 | 95 ± 9 | |
| CD45RO+CCR7+ | 57 ± 17 | 65 ± 13 | 66 ± 18 | 60 ± 13 | |
| CD45RO+CD46+ | 100 ± 1 | 99 ± 1 | 99 ± 1 | 100 ± 1 |
Results are expressed as percentage of positive cells ± s.d. To analyse the expression of different surface markers four-colour FACS stainings were performed. The percentages were calculated from the total amount of CD4+ T cells positive for the specific antibodies as analysed by winmdi 2·8 software.
a
Statistical analysis was performed by anova with Tukey–Kramer adjustment for multiple comparisons.