TABLE 2.
Summary of microsatellites tested in SK-N cellsa
| Locusb | Repeat unit | No. of repeat units (GenBank) | No. of interrup- tionsc | Length of longest uninterrupted tract (repeat units) | Repeat length of alleles in SK-Nd (repeat units) | Altered in D181A cells |
|---|---|---|---|---|---|---|
| dyn | CAG | 10 | 1 | 10 | 10, 10 | No |
| HD | CAG | 21 | 0 | 21 | 23, 26 | Yes |
| Sca1 | CAG | 30 | 2 | 15 | NDe | No |
| TBP | CAG | 38 | 4 | 18 | 37, 38 | No |
| ACTC | CA | 25 | 0 | 25 | 24, 26 | Yes |
| D6S264 | CA | 20 | 1 | 17 | ND | No |
| D6S305 | CA | 21 | 0 | 21 | 14, 13 | No |
a
Repeat regions at each of the sites indicated were amplified and sequenced in SK-N-MC parent and FEN-transformed cells.
b
dyn, prodynorphin; Sca1, spinocerebellar ataxia type 1 gene; TBP, TATA binding protein; ACTC, D6S264, and D6S305, CA microsatellite markers (20).
c
Imperfections in the repeating units were sometimes present, and the number of these at each locus as well as the length of the longest uninterrupted tract is indicated.
d
The length as determined by amplification in SK-N-MC cells at some loci differed from that of the GenBank sequence.
e
ND, not determined.