Table 2.

Effect of IFNγ on marker expression in normal and IBD LPLs

	HLA-DR (% positive cells)		MHC cl I (RFI)		CD86 (% positive cells)
	W/o IFNγ	W/IFNγ	W/o IFNγ	W/IFNγ	W/o IFNγ	W/IFNγ
Nl T cells	10 ± 3	11 ± 3	50 ± 10	170 ± 22*	1 ± 1	1 ± 1
Nl ECs	3 ± 2	9 ± 2*	41 ± 8	162 ± 10*	1 ± 1	5 ± 1*
IBD T cells	44 ± 9	47 ± 8	59 ± 11	180 ± 19*	6 ± 2	6 ± 3
IBD ECs	34 ± 8	59 ± 9*	51 ± 9	179 ± 12*	9 ± 2	19 ± 3*

LPLs were obtained from 4 normal colons, 4 with UC, and 4 with CD and cultured for 24 h with or without IFNγ (100 ng/ml) in the absence of other stimuli. The expression of the markers listed was determined by double immunofluorescence using CD2-FITC and PE-conjugated mAbs against each marker. The percentage positive cells is shown (mean ± SD) for HLA-DR and CD86 expression, while the RFI is listed for MHC class I since it is found on all cells. Since data for UC and CD did not differ, these values were pooled. The asterisks mark those values that significantly differed in the presence or absence of IFNγ.

^*P < 0·05