Table 3.
T-APCs from IBD colonic LPLs: activation antigens and stimulatory activity
| IL-2 | IL-2 + IL-10 | |
|---|---|---|
| Activation antigens (% positive cells (RFI)) | ||
| MHC class I | 100 (65 ± 8) | 100 (53 ± 9) |
| HLA-DR | 28 ± 2% (6 ± 1) | 19 ± 3%* (5 ± 1) |
| CD80 | 2 ± 1 (2 ± 1) | 2 ± 1 (2 ± 1) |
| CD86 | 6 ± 1 (2 ± 1) | 1 ± 1* (2 ± 1) |
| Stimulatory effect (Specificity of MAb added) Stimulation index | ||
| IgG | 8·9 ± 1·0 | 6·8 ± 0·9* |
| MHC class I | 8·5 ± 1·2 | 6·5 ± 1·0* |
| HLA-DR | 5·5 ± 0·8† | 4·2 ± 0·8† |
| CD80 | 8·6 ± 1·2 | 6·3 ± 0·8* |
| CD86 | 5·4 ± 0·8† | 4·4 ± 1·1† |
| DR, CD80, CD86 | 3·3 ± 0·9‡ | 2·9 ± 0·6‡ |
LPLs from 8 IBD patients (4 with UC and 4 with CD) were cultured for five days with IL-2 in the presence or absence of IL-10 (each at 10 ng/ml). The data represent a mean ± SD from all patients. The percentages of CD2+ cells expressing each antigen are shown followed by the RFI in parentheses. The asterisks identify values that significantly differed with and without IL-10(*P < 0·05). The T-APCs were irradiated, then cultured with allogeneic T cells depleted of conventional APCs. Proliferation in this MLR was measured by 3HTdr incorporation on day 5 in the presence of blocking Abs. The results are expressed as a stimulation index, that is, the counts per min (cpm) for the test divided by the cpm of T cells in medium alone. The asterisks identify values that significantly differed with and without IL-10 (*P < 0·05). The triangles identify test values that significantly differed from those obtained with irrelevant IgG († = P < 0·05; ‡ = P < 0·01).