Table 4.
T-APCs from PBLs | T-APCs from LPLs | |||
---|---|---|---|---|
IL-2 | IL-2 ± IL-10 | IL-2 | IL-2 ± IL-10 | |
Activation antigens (% positive cells (RFI)) | ||||
MHC class I | 100% (55 ± 8) | 100% (49 ± 8) | 100% (51 ± 9) | 100% (49 ± 10) |
HLA- DR | 42 ± 5 (6 ± 2) | 29 ± 4* (5 ± 2) | 39 ± 4 (6 ± 2) | 21 ± 5* (5 ± 1) |
CD80 | 3 ± 1 (3 ± 1) | 2 ± 2 (2 ± 1) | 4 ± 1 (3 ± 1) | 3 ± 2 (2 ± 1) |
CD86 | 8 ± 2 (2 ± 2) | 3 ± 1* (2 ± 1) | 9 ± 2 (2 ± 1) | 4 ± 1* (2 ± 1) |
Stimulatory activity (Specificity of MAb added) Stimulation index | ||||
IgG | 7·6 ± 1·1 | 4·9 ± 1·2* | 8·1 ± 1·1 | 5·9 ± 1·8* |
MHC class I | 7·5 ± 1·0 | 4·7 ± 1·1* | 7·9 ± 1·0 | 5·5 ± 1·4* |
HLA-DR | 5·8 ± 0·9† | 3·6 ± 0·9† | 5·5 ± 1·1† | 3·8 ± 0·9† |
CD80 | 7·3 ± 1·1 | 4·4 ± 1·1* | 7·1 ± 0·9 | 4·6 ± 0·9* |
CD86 | 5·6 ± 1·1† | 3·1 ± 0·8† | 5·1 ± 1·1† | 3·3 ± 0·9† |
DR, CD80, CD86 | 4·9 ± 1·0‡ | 2·8 ± 1·0† | 3·1 ± 0·8‡ | 1·8 ± 0·9‡ |
Normal PB T cells and LPLs (depleted of APCs) from 4 individuals were repeatedly activated with both allogeneic irradiated feeder PBLs and IL-2 in the presence or absence of IL-10 (each at 10 ng/ml). The data represent a mean ± SD from all patients. The percentages of CD2+ cells expressing each antigen are shown followed by the RFI in parentheses. The asterisks identify values that significantly differed with and without IL-10(*P < 0·05). The proliferation of allogeneic T cells (depleted of APCs) in response to irradiated T-APCs was measured by 3HTdr incorporation in the presence of blocking Abs. The results are expressed as the stimulation index. The asterisks identify values that significantly differed with and without IL-10 (* P < 0·05). The bars identify test values that significantly differed from those obtained with irrelevant IgG(† = P < 0·05; ‡ = P < 0·01).