Fig. 1.

Typical example illustrating the peripheral blood dendritic cell (DC) composition of a healthy volunteer. First, leucocytes were gated using their forward and side-scatter characteristics (Fig. 1a, left panel). Next, DCs were identified as leucocytes that stained negative for lineage and positive for HLA-DR (a, middle panel). The myeloid and plasmacytoid DC subpopulations were then distinguished based on their expression of CD123 & CD11c (a, right panel). The maturation status of the myeloid (filled histograms) and plasmacytoid (grey histograms) DC subsets was determined by analysing the expression of CD83 (b) and CCR7 (c).