Fig. 5.

CpG-oligodeoxynucleotides (ODN) stimulates intracellular interferon (IFN)-γ expression. Mice infected intratracheally with Cryptococcus neoformans were treated with 20 µg/mouse of CpG- or CNT-ODN on days −3, 0, 3, 7 and every 7 days after infection. The lung leucocytes prepared on days 7 (a) and 14 (b) post-infection were stained with fluoroscein isothyocyanate (FITC)-anti-IFN-γ MoAb and phycoerythrin (PE)-anti-CD4 or -CD8 MoAb and analysed by flow cytometry. Each number indicates the proportion of each subset. The experiments were repeated twice with similar results.