Fig. 3.

Phosphor-images showing the effects of pneumolysin (Ply 8·37 and 41·75 ng/ml) on nuclear translocation of NFκB in human neutrophils in the absence and presence of (a) 10 m m EGTA or (b) 5 and 10 µg/ml docosahexaenoic acid (DHA). Neutrophils were treated with pneumolysin for 15 min at 37°C in the absence and presence of EGTA or DHA and the nuclear extracts then analysed by electrophoretic mobility shift assay.