Table 2.

Effects of addition of EGTA to the neutrophil-suspending medium on pneumolysin-mediated influx of Ca²⁺

System	Amount of cell-associated 45Ca2+ (pmoles/107 cells/5 min)
Neutrophils only	179 ± 6
Neutrophils + 10 m m EGTA	132 ± 5
Neutrophils + 8·37 ng/ml pneumolysin	2031 ± 91*
Neutrophils + EGTA + pneumolysin	160 ± 10

The results are expressed as the mean values ± s.e.m. of a single representative experiment (2 in the series) with 5 replicates for each system.

^*P < 0·05 for comparison with each of the other systems.