Fig. 2.
Effect of p50 antisense oligonucleotide on E. coli-induced cytokine and nitric oxide production. U87-MG astrocytes were transfected with either p50 antisense (AS) or sense (S) oligonucleotides using DOTAP. The transfected cells were then infected with E. coli for 24 h. Protein levels of each cytokine in culture supernatants were determined by ELISA. The levels of NOx (i.e. NO2– and NO3–) were determined, using the Griess reaction and nitrate reductase. Data are mean ± SEM (n = 5). *values with E. coli+ antisense oligonucleotide sequence that are significantly different from those of E. coli alone (P < 0·05).