Fig. 5.

The effects of IκBα, IKKβ and NIK super-repressors on reporter gene activation in E. coli-infected U87-MG astrocytes. U87-MG cells were transfected with pIL8-, pMCP-1, or p2x NF-κB-luciferase transcriptional reporters together with IκBα-AA, IKKβ-AA, or NIK-AA expression vectors, as indicated. After 48 h, the transfected cells were infected with E. coli (▪) or TNF-α (20 ng/ml, □) for 6 h. Data are expressed as mean fold induction ± SEM in luciferase activity relative to noninfected controls (n = 7).