Table 2.

Activation of IL-8-luciferase reporter genes in E. coli-infected astrocytes.^*

	IL-8-luciferase activity
	E. coli-infected	TNF-α-stimulated
None	6·21 ± 1·88†	10·22 ± 1·11
TRAF2	3·12 ± 0·64‡	1·85 ± 0·64‡
TRAF5	4·91 ± 1·21‡	3·36 ± 0·46‡
Control plasmid	6·67 ± 2·77	11·17 ± 2·33

^*U87-MG cells were transfected with IL-8-luciferase transcriptional reporters together with expression vectors for superrepressors of TRAF2 or TRAF5, as indicated. After 48 h, cells were infected with E. coli or stimulated with TNF-α (20 ng/ml) for 6 h.

^†mean fold induction ± SEM in luciferase activity relative to noninfected controls (n = 7).

^‡Significantly different from values for nontransfected cells infected with E. coli or stimulated with TNF-α, respectively (P < 0·05).